SFC 2012 Short Course

Date: October 3, 2012
Time: 12:30pm to 5:00pm
Place: Square Brussels Meeting Centre, Brussels, Belgium
Instructors: Larry Miller (Amgen) and Larry Taylor (Virginia Tech)

Taylor Bio: Larry T. Taylor is Emeritus Professor of Chemistry, Virginia Tech (Blacksburg, VA). He is a member of the editorial boards for the Journal of Chromatographic Science, Chromatographia, and the Journal of Supercritical Fluids. He was a member of the organizing committee for the 4th through the 10th International Symposia on SFC/SFE. More recently he has served as the Co-Chair of the Scientific Committee for SFC 2008 (Zurich), SFC 2009 (Philadelphia), SFC 2010 (Stockholm), and SFC 2011 (New York City). Larry is the author or co-author of approximately 400 peer reviewed publications and 12 patents. He presently serves as co-teacher of short courses addressed to SFC and SFE.

Miller Bio: Larry Miller is a Principal Scientist in the Discovery Analytical Sciences group at Amgen in Cambridge, MA. He graduated with a BS degree from the University of Illinois in Urbana-Champaign in 1984 and earned a MS from Roosevelt University in 1990. In 1984, he joined Searle in suburban Chicago as an analytical chemist. He spent twenty years performing small molecule achiral and chiral purifications at the mg to multi-kg scale, utilizing preparative HPLC, steady state recycle (SSR) and simulated moving bed (SMB) chromatography. He managed a group of ten scientists within a purification center of excellence at Searle/Pharmacia, providing purification support to three R&D sites. In 2004, Larry joined Amgen. At Amgen he is responsible for discovery and early development purification support utilizing preparative SFC and HPLC.

Agenda:

12:30pm -1:30pm "Fundamentals of Analytical SFC"

Properties of supercritical fluids, packed and open tubular columns, efficiency, CO2, column pressure drop, speed, retention, pumping, column back pressure, safety issues, UV-MS-ELSD detection

1:35pm - 2:35pm "Chiral Chromatography with Supercritical Fluids"

Introduction to chirality, chiral recognition, advantages of SFC vs. HPLC, stationary phase and mobile phase screening, column coupling, rapid method development strategies

Break

2:50pm - 3:55pm "Methods Development in Achiral Packed Column SFC"

Modifiers/retention/selectivity, additives, polar stationary phases, SFC employing HILIC-like conditions, ion pair SFC, reversed phase SFC, generic pcSFC methods, mixed-mode retention mechanisms, applications directed to phospholipids, peptides, pharmaceuticals

4:00pm - 5:00pm "Preparative Supercritical Fluid Chromatography"

Introduction to small molecule purification, HPLC vs. SFC; advantages and disadvantages, effect of solubility in preparative SFC, preparative sample injection options, chiral and achiral preparative SFC, mass direction purification, preparative SFC safety concerns